Dates
Please inquire for actual course date.
Molecular Biology Basic Course
Content & Learning Objective
Molecular biology focuses on the structure, biosynthesis and function of DNA and RNA on the molecular level and how these interact among themselves and with proteins. Molecular biology techniques are essential for modern biological and medical research and the range of these techniques extends from PCR, cloning, mutagenesis, recombinant expression etc. to phage display or yeast two hybrid systems. This course will give you an introduction to DNA and RNA standard techniques.
The theoretical part includes:
- Basic knowledge of Molecular Biology
- Structure of macromolecules
- Principles of cloning
- Cloning vectors
- Restriction enzymes
- RNA isolation and reverse transcription
- Polymerase Chain Reaction (PCR)
- Gene regulation and gene expression
The practical part includes:
- Plasmid preparation
- Determination of DNA concentrations
- RNA isolation and reverse transcription
- Polymerase Chain Reaction (PCR)
- DNA gel electrophoresis, documentation, gel extraction
- Restriction digest, vector dephosphorylation, ligation of DNA
- Generation of competent cells and their transformation
- Preparation of agar plates with and without antibiotics
- Over night cultures and selection on plates, blue white selection
Target Group
Technical and scientific staff members without any previous knowledge or possessing only basic knowledge of molecular biology.
Teacher
Dr. Britt Lemke studied biology in Potsdam. In 2004, she received her Ph.D. from the Max-Delbrück-Center for Molecular Medicine in the area of hematopoietic development. After this, she held a postdoc position at the neurosurgical university clinic Heidelberg with the topics proteomics, angiogenesis, tumor development, and immuno therapy of brain tumors. In 2008, she joined the PromoCell Academy as lab manager and lecturer.
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