Quality Management in Cell Culture Labs

Content & Learning Objective

Defining standards in cell culture is usually perceived as being complicated and therefore, establishing guidelines is postponed or omitted. However, standards are an indispensable prerequisite for gaining reliable, objective, and reproducible results and consistently avoiding contamination. In this course, you will be trained to perform cell culture routine methods according to current quality guidelines. Even though GMP and GLP are not a central topic in this course, standardization will be presented e.g., by generating SOPs and by recommendations in line with GLP and GMP regulations.

Topics in this theory course are:

• Standardization of cell culture routine methods such as passaging, thawing, and freezing of cells
• Contamination control with a focus on Mycoplasmas
• Quality of reagents in cell culture, e.g., FCS
• Prevention of cross contaminations
• Methods for characterizing cells
• Assurance of lasting quality of cell lines
• Cell banking

Target Group

Technical and scientific staff members with cell culture experience who want to improve the quality of their cell cultures and the reliability of their results but do not work under GMP or GLP.

Teacher

Dr. Silke Busch started her studies in biology at the Technical University Braunschweig and received her PhD from the Institute of Biochemistry at the University Hospital Lübeck. Then, she joined the University Hospital in Tübingen and focused first on research in the field of molecular pathology and later on the field of dermatologic oncology. Since 2007, she has worked as a Specialist for 3D-cell culture models in the research and development department of PromoCell GmbH.