Program
 
 

Quality Management in Cell Culture Labs

Content & Learning Objective

Defining standards in cell culture is usually perceived as being complicated and therefore, establishing guidelines is often postponed or omitted. However, standardization is an indispensable prerequisite for gaining reliable, objective, and reproducible results and consistently avoiding contamination. In this course, you will be instructed on how to perform routine cell culture methods according to current quality guidelines. Even though GMP and GLP are not a central topic in this course, standardization will be presented e.g., by generating SOPs and by using recommendations in line with cGLP/cGMP regulations.

Topics in this theory course are:

  • Standardization of cell culture routine methods such as passaging, thawing, and freezing of cells
  • Contamination control with a focus on mycoplasma
  • Quality of reagents in cell culture, e.g., FCS
  • Prevention of cross contaminations
  • Methods for characterizing cells
  • Assurance of lasting quality of cell lines
  • Cell banking

Target Group

Technical and scientific staff members with cell culture experience who want to improve the quality of their cell cultures and the reliability of their results, but do not work under GMP or GLP.

Teacher

Dr. Nicole Kühl studied Biology at the Technical University of Karlsruhe and the University of Bremen. In 1999 she completed her PhD in cell and molecular biology studying the cell cycle and heat shock proteins. Dr. Kühl then began her post-doctorate work at the Academic Hospital Groningen in the Netherlands, where she specialized in research related to Multiple Sclerosis and worked with primary glial cells. In 2005, she continued her work at the Jacobs University Bremen, where she led a research group and lectured in biochemistry and cell biology. From 2008 to 2011, Dr. Kühl was head of the PromoCell Academy and taught cell culture courses. Since 2012, she is a freelance trainer and consultant in the area of cell culture quality control (www.incelligence.de).