Sterile Working Techniques

Content & Learning Objective

This course provides you with the theoretical and practical basics of sterile working techniques so that your daily work with cell cultures will become safer and more efficient. You will gain crucial knowledge about the functional principles of your most important tool – the clean bench. These principles determine certain rules for working under the bench, which we will practice intensively in the course.

The theoretical part includes:

• Sterile working techniques
• Functional principles of clean benches
• Contaminations in cell cultures

The practical part includes:

• Working with cells under the clean bench
• Thawing of cells
• Subcultivation of cells

Target Group

Technical and scientific staff members with no previous cell culture experience or little cell culture experience.

Teacher

Dr. Nicole Kühl began her academic career at the Technical University of Karlsruhe in 1988. In 1991, she transferred to the University of Bremen where she completed her Ph.D. in cell and molecular biology studying the cell cycle and heat shock proteins. Dr. Kühl then began her post-doctorate work at the University and University Hospital of Groningen in the Netherlands, where she specialized in research related to multiple sclerosis and worked with primary glial cells. In 2005, she continued her work at the Jacobs University Bremen, where she led a research group and lectured in biochemistry and cell biology. Since 2008, Dr. Kühl has been the head of the PromoCell Academy and has taught cell culture courses.

Recommended advanced courses

Cell Culture Trouble Shooting, Quality Management in Cell Culture Labs