Cell Culture Basic Course

Content & Learning Objective

This course will teach you basic knowledge or improve your existing expertise, so that your everyday work with cell culture will become safer and more efficient. Most of the time, beginners in this field can only acquire the basic knowledge they need by reproducing what their colleagues do, and standards which are vital for the comparability of the test results are frequently missing. You will have sufficient time to acquire practical experience during the training and additionally, we will introduce you to practical standards and documentation as well as cell banking.

The theoretical part includes:

• Sterile working techniques and contaminations
• Routine methods in cell culture
• Ingredients of culture media
• Cell counting and viability testing
• Cell banking

The practical part includes:

• Introductory session: sterile working techniques
• Trypsinization, freezing, and thawing of cells
• Medium exchange of adherent and suspension cells
• Isolation of primary human endothelial cells

Target Group

Technical and scientific staff members with no previous cell culture experience or little cell culture experience.

Teacher

Dr. Nicole Kühl studied Biology at the Technical University of Karlsruhe and the University of Bremen. 19999 she completed her Ph.D. in cell and molecular biology studying the cell cycle and heat shock proteins. Dr. Kühl then began her post-doctorate work at the Academic Hospital Groningen in the Netherlands, where she specialized in research related to multiple sclerosis and worked with primary glial cells. In 2005, she continued her work at the Jacobs University Bremen, where she led a research group and lectured in biochemistry and cell biology. From 2008 to 2011, Dr. Kühl was the head of the PromoCell Academy and taught cell culture courses. Since 2012, she is a freelance trainer and consultant in the area of cell culture quality control and runs the cell culture website InCelligence.de.