ELISA Advanced Course

Content & Learning Objective

In this ELISA course, you will be introduced to the possibilities of ELISA techniques in theory and practically, and you will develop the competence to establish these techniques in your laboratory. The theoretical module covers the possibilities and parameters of different methods while in the practical parts, you will use these to solve an analytical problem (determination of the degree of contamination in a product using a biochemical assay). In order to do so, you independently establish an ELISA system and you optimize it under assistance. Thereafter, a systematical error analysis is discussed and possible short-comings of the system are identified and removed.

The course covers:

• Short introduction to ELISA techniques and definition of important vocabulary
• Assay formats
• Components of ELISA systems
• Generation of antibody conjugates
• Possibilities for optimization of components
• Choice of optimal ELISA concepts
• Optimization of robustness and stability of the test system
• Development strategy – from assessment of demand to the final assay
• Determination of capacity – verification and validation of an ELISA
• Trouble shooting by systematic error analysis
• Standardization

Target Group

Technical and scientific staff members, who want to establish or optimize new ELISA techniques or who want to develop an ELISA for sales.

Teacher

Dr. Michael Oed studied biology at the university Mainz and received his Ph.D. for studying the interaction of viral and eukaryotic DNA. From 1991 to 2003 he was project leader at Byk-Sangtec Diagnostica (ALTANA) where he developed immunoassays. After that, he joined mtm laboratories as head of development for immunodiagnostic methods for early diagnosis of cancer. Besides this, Dr. Oed works as a consultant for development and production of IVD products under cGMP.