Cell Culture for Non-Biologists

Content & Learning Objective

Cell culture has also become an important source of data for related disciplines, making it increasingly important for users without training in cell biology to understand and be able to apply the complex methods of this field. This course familiarizes you with the basics of cell culture, covering both theory and practice.

The theoretical part includes:

  • Structure and function of eukaryotic cells and basic cell biology
  • Overview of frequently used cell lines and what they are good for
  • Basics of sterile methods for preventing contamination
  • Media, additives, and culture vessels

The practical part comprises:

  • Use of sterile techniques in cell culture
  • Basic techniques for culturing cell lines

Manual and automated counting of live cells

Target Group

Technical and scientific staff without training in cell biology who have limited or no knowledge of cell culture and want to familiarize themselves with the theory and practice of cell culture work


Associate professor Dr. Rüdiger Arnold  teaches at the University of Heidelberg as a lecturer in several life science education programs. Dr. Arnold studied biology in Giessen and worked as a postdoctoral fellow at the Max - Planck - Institute of Physiology and Clinical Research in Bad Nauheim. Afterwards he joined the German Cancer Research Center and habilitated at the medical faculty of the Ruprecht-Karls - University of Heidelberg in immunology where he worked in the area of activation, differentiation, and apoptosis in leukocytes and lymphoma. Between 2011 and 2015 he headed the student research laboratory of the Heidelberg Life - Science Lab at the German Cancer Research Center.

Recommended advanced courses

Cell Culture Basic Course