Detection, Prevention and Elimination of Mycoplasma

Content & Learning Objective

This course teaches you how to detect contamination of cell cultures with mycoplasma and other bacteria at an early stage, plus practical methods for preventing and eliminating these in your everyday work. The implications for scientific and industrial work are discussed, in addition to different contamination paths and detection methods. Ways to remove mycoplasma from cultures are also presented.

The theoretical part includes:

  • Causes of contamination by mycoplasma
  • How contamination can affect cell cultures
  • Ways to prevent, detect, and eliminate contamination
  • Methods for detecting mycoplasma


The practical part comprises:

  • Mycoplasma detection with PCR

Target Group

Technical and scientific staff with basic knowledge of cell culture


Associate professor Dr. Rüdiger Arnold  teaches at the University of Heidelberg as a lecturer in several life science education programs. Dr. Arnold studied biology in Giessen and worked as a postdoctoral fellow at the Max - Planck - Institute of Physiology and Clinical Research in Bad Nauheim. Afterwards he joined the German Cancer Research Center and habilitated at the medical faculty of the Ruprecht-Karls - University of Heidelberg in immunology where he worked in the area of activation, differentiation, and apoptosis in leukocytes and lymphoma. Between 2011 and 2015 he headed the student research laboratory of the Heidelberg Life - Science Lab at the German Cancer Research Center.