ELISA Basic Course

Content & Learning Objective

In this course, you will learn the theory and practice of ELISA techniques, from sample preparation and processing of multiwell plates to computerised evaluation and quality control of the results.

The theoretical part covers:

  • ELISA basics
  • Sample material and sample preparation
  • ELISA components: choice of antibodies, plastic ware, coating, standards, etc.
  • Standards and controls
  • Quality parameters: detection limit, accuracy, reproducibility, linearity, recovery
  • Evaluation of data and quality control
  • Trouble shooting

The practical part covers:

  • Performance of a Sandwich-ELISA
  • Performance of a competitive ELISA

Target Group

Technical and scientific staff members, who are interested in establishing and using the ELISA technique in the laboratory.


Dr. Peter Schröder studied biology at Heinrich Heine University in Düsseldorf and also did his doctorate there at the Institute of Physiological Chemistry. After stints abroad in Milwaukee and Stockholm, he joined the research staff of the university’s Research Institute for Environmental Medicine. He was involved in developing ELISAs for quantifying sexual hormone in pelagic fish and gained experience with a wide range of commercially available ELISAs for oxidative stress, dermatology and geriatric research. Since 2011, Dr. Schröder has worked as an independent teacher, coach and consultant.

Recommended advanced courses

ELISA Advanced Course