General Lab Course on Cell Culture

Content & Learning Objective

In recent decades, cell culture has grown into a broad and increasingly complex field. This course gives you an overview of the various types of cell culture and their applications. The properties and sterile handling of primary cells and cell lines are discussed, as well as the basics of three-dimensional cell culturing, standardization methods, and the ingredients of media and reagents.

The theoretical part comprises:

  • Cell lines and primary cells
  • Basics of three-dimensional cell culture
  • Sterile techniques
  • Contamination of cell cultures
  • Vitality tests
  • Overview of media, additives, and vessels used in cell culture
  • Basics of standardization in cell culture
  • Basics of cryo-conservation and cell banking

The practical part comprises:

  • Sterile cell culture techniques
  • Cultivation of cell lines and primary cells
  • Isolation of primary human cells
  • Cell vitality assays

Target Group

Technical and scientific staff with a basic knowledge of cell culture wishing to get acquainted with the methods used


Associate professor Dr. Rüdiger Arnold  teaches at the University of Heidelberg as a lecturer in several life science education programs. Dr. Arnold studied biology in Giessen and worked as a postdoctoral fellow at the Max - Planck - Institute of Physiology and Clinical Research in Bad Nauheim. Afterwards he joined the German Cancer Research Center and habilitated at the medical faculty of the Ruprecht-Karls - University of Heidelberg in immunology where he worked in the area of activation, differentiation, and apoptosis in leukocytes and lymphoma. Between 2011 and 2015 he headed the student research laboratory of the Heidelberg Life - Science Lab at the German Cancer Research Center.

Recommended advanced courses

Cell Culture: Advanced Course; Flow Cytometry, Spheroid Culture, Skin Models, Induced Pluripotent Stem Cells: Customized Cell Models; Transfection and Reporter Gene Analysis; Cell Viability, Proliferation and Toxicity Tests; Use and Optimization of Transfection Methods; Fluorescence Microscopy of Live Cells; Industrial Cell Culture Technology