Cell Culture Basic Course

Content & Learning Objective

This course will provide basic knowledge and build upon your existing expertise, so that your daily cell culture work becomes safer and more efficient. Beginners to cell culture often get by with only a basic and limited knowledge acquired from copying a colleague. High standards required for reproducibility and accuracy are frequently missing. During this course, you will have sufficient time to acquire practical experience and in addition, we will introduce you to practical standards, documentation and cell banking.

The theoretical part includes:

• Aseptic techniques and contaminations
• Routine methods in cell culture
• Ingredients of culture media
• Cell lines and primary cells
• Cell counting and viability testing
• Cell banking

The practical part includes:    

• Sterile working techniques
• Trypsinization, freezing, and thawing of cells
• Medium exchange for adherent and suspension cells
• Isolation of primary human endothelial cells

Target Group

Technical and scientific staff members with no previous cell culture experience or little cell culture experience.

Teacher

Priv.-Doz. Dr. Rüdiger Arnold studierte Biologie in Gießen und arbeitete danach als Postdoktorand am Max-Plank-Institut für Physiologie und Klinische Forschung in Bad Nauheim. Im Anschluss wechselte er ins Deutsche Krebsforschungszentrum und habilitierte an der Medizinischen Fakultät der Ruprecht-Karls-Universität Heidelberg im Fach Immunologie. Dort beschäftigte er sich mit der Aktivierung, Differenzierung und Apoptose in Leukozyten und bei Lymphomen. Von 2011 bis 2015 leitete er das Schülerforschungslabor des Heidelberger Life-Science Lab am Deutschen Krebsforschungszentrum. Gegenwärtig ​lehrt er an der Universität Heidelberg und ist ​als Dozent ​bei biowissenschaftlichen Weiterbildungsprogrammen tätig.

Recommended advanced courses

Cell Culture Trouble Shooting, Quality Management in Cell Culture Labs