Programm
 
 

Flow Cytometry

Content & Learning Objective

In this course, you first receive a detailed introduction to flow cytometry, which includes florescence-activated cell sorting (FACS). Then we thoroughly explain how a flow cytometer works and its most common applications, such as checking the quality of cell cultures. Some of the topics discussed in the first (theoretical) part are later illustrated in a practical demonstration.

In the practical demonstration, the following parameters are measured in various cell cultures and samples:

  • Cell phenotyping
  • Multicolor analysis
  • Determining whether cells are dead or alive (vital)
  • Volumetric cell counting

The course includes plenty of time for hands-on practice with the demo equipment and answering your questions.

Target Group

Technical and scientific staff with good basic knowledge of cell culture and flow cytometry. Basic familiarity with flow cytometer is advantageous but not required for attending this course.

Teacher

Dr. Steffen Schmitt studied biology at Heidelberg University, going on to complete a doctorate in immunology. Later he built and then headed the flow cytometry service unit at the University of Mainz. Since 2007, Dr. Schmitt has been in charge of the flow cytometry service unit of the central imaging and cytometry unit of the German Cancer Research Center in Heidelberg.

Recommended advanced courses

Fluorescence Microscopy of Live Cells