Programm
 
 

Induced Pluripotent Stem Cells: Customized Cell Models

Content & Learning Objective

In this course, you learn more about the generation, characterization, quality checking, and use of human induced pluripotent stem cells (iPS cells). In the practical part, you are shown methods for culturing, evaluating and distinguishing iPS cells. The theoretical part includes:

  • What are iPS cells?
  • How do you get iPS cells
  • How do you culture human pluripotent stem cells, and what characterizes them?
  • How do you check the pluripotency of human iPS cells?
  • How do you tell human iPS cells apart?
  • Examples of how human iPS cells are used to study diseases

The practical part includes:

Culturing of human iPS cells

  • Cryo-conservation of human iPS cells
  • Evaluation of cell morphology
  • Pluripotency test
  • Telling human iPS cells apart

Target Group

Technical and scientific staff with good basic knowledge of cell culture

Teacher

Privatdozent Dr. Katrin Streckfuss-Bömeke studied biology at the University of Göttingen and obtained her doctorate in genetic microbiology at the Institute of Microbiology and Genetics there with a dissertation on protein interactions in yeast as a model organism. In 2006 she switched from yeast to studying murine and human stem cells in the stem cell lab of the cardiology/pulmonology department of the Göttingen University Clinic. While there she intensively studied characterizing pluripotency analysis and methods for differentiating induced pluripotent stem cells (iPS cells). Currently she is a group leader at the university’s medical school, where her focus is on modeling and studying the mechanisms of various cardiac diseases using patient-specific iPS cells. In 2018 she earned Habilitation, Germany’s highest merit-based academic qualification, with a thesis on pluripotent stem cells in cardiovascular research.