Process Technology for Cell Culture Bioreactors

Content & Learning Objective

The choice of technology for a bioreactor depends mainly on what it will be used for. Batch culturing is safe and easy, but can only produce small amounts of cells and their products. A fed batch system can increase the yield significantly, but calls for special feeding strategies. Continuous chemostat cultures are suitable mainly for identifying the kinetic metrics of cells. Perfusion cultures with cell retention have potential for producing large quantities of cells and their products. This course talks about various process flow strategies and their relative advantages and drawbacks for different tasks. Batch, fed batch, and chemostat simulations are performed on a virtual bioreactor to help develop strategies for optimally managing them.

The main focuses of this course include:

  • Bioreactor technologies for cell culture (batch, fed batch, chemostat, perfusion)
  • Evaluation of culture data
  • Designing experiments
  • Identification of metrics for cell culture processes

Simulation of strategies by participants using a PC

Target Group

Technical and scientific staff with good basic knowledge of cell culture and cell biology and basic knowledge of bioreactor technology


Prof. Dr.-Ing. Ralf Pörtner studied chemical technology at Dortmund University, doing his doctorate in the Department of Mechanical and Process Engineering. After pursuing postdoctoral studies at the University of Tsukuba in Japan, he returned to Germany to take the roles of Head Engineer and Head of the Cell Culture Technology and Tissue Engineering working group at the Hamburg University of Technology. After achieving the highest German merit-based academic qualification, Habilitation, Dr. Pörtner was granted Privatdozent status, thus licensing him to teach at the university level. Since 2010 he has been an honorary professor at the Mittelhessen University of Applied Sciences in Giessen. Dr. Pörtner is currently one of the coordinators of the regeneration, implants and medical technology research department in Hamburg and also serves on the board of the Forschungszentrum Medizintechnik (FMTHH). His research focuses include developing bioreactors, especially for cell culture and microbial reactions, model-assisted control and regulation approaches, and tissue engineering.